Cadmium-113 Fourier transform nuclear magnetic resonance of cadmium(II) carbonic anhydrases and cadmium(II) alkaline phosphatase

113Cd nuclear magnetic resonance of mammalian erythrocyte carbonic anhydrases.

'13Cd NMR of the "3Cd(II)-substituted zinc metalloenzymes, human and bovine erythrocyte carbonic anhydrases, is reported. The high activity isozymes, human erythrocyte carbonic anhydrase, isozyme C (HCAC) and bovine erythrocyte carbonic anhydrase, isozyme B (BCAB), are characterized by relatively narrow '13Cd resonances around 220 ppm to lowfield of Cd(C104)Z. These resonances are relatively in...

Fourier Transform Multiple Quantum Nuclear Magnetic Resonance

The excitation and detection of multiple quantum transitions in systems of coupled spins offers, among other advantages, an increase in resolution over single quantum n.m.r. since the number of lines decreases as the order of the transition increases. This paper reviews the motivation for detecting multiple quantum transitions by a Fourier transform experiment and describes an experimental appr...

31P nuclear magnetic resonance of phosphoenzyme intermediates of alkaline phosphatase.

Covalent (E-P) and noncovalent (E X P) phosphoenzyme intermediates exist on the reaction path of alkaline phosphatase of Escherichia coli. Zn(II) and Cd(II) alkaline phosphatases both form E-P and E X P from inorganic phosphate. These intermediates show well separated 31P NMR resonances in slow chemical exchange with respect to each other and to unbound phosphate. The 31P signals of E X P of al...

Carbon-13 Fourier transform nuclear magnetic resonance. II. Ribonuclease.

High-resolution Fourier transform nuclear magnetic resonance was used to observe the natural abundance (13)C spectrum of a 0.02 M solution of bovine pancreatic ribonuclease A. Peaks assignable to only three carbons were easily observed after 10 hr of signal averaging. A number of tentative assignments were made. As expected, the (13)C spectrum was appreciably richer in detail than the correspon...

Nuclear Magnetic Resonance Fourier Transform Spectroscopy ( Nobel Lecture ) 1 Richard